Objective To investigate whether hemospermia is involved in the production of antisperm antibodies(ASA).Methods A total of 40 males with hemospermia were enrolled in this study. These males were divided into 3 groups according to their fertile status: 18 cases were patients who visited the infertility clinic(group A), 8 had fertility(group B), and 14 did not know their fertile status(group C). Semen samples were collected and ASA levels were detected by the direct immunobead test(D-IBT). Leukocytes in semen were assessed by the peroxidase assay.Results None of these males with hemospermia were found to display significant levels of ASA in the 3 groups. The number of cases who had increased levels of leukocytes in the semen samples of groups A, B and C were 15(83.3%), 6(75.0%) and 12(85.7%),respectively.Conclusion Hemospermia is not associated with the production of ASA. It is worth noting that the leukocyte levels are increased in semen of males with hemospermia. Objective To investigate whether hemospermia is involved in the production of antisperm antibodies (ASA). Methods A total of 40 males with hemospermia were enrolled in this study. These males were divided into 3 groups according to their fertile status: 18 cases were patients who visited The infertility clinic (group A), 8 had fertility (group B), and 14 did not know their fertile status (group C). Semen samples were collected and ASA levels were detected by the direct immunobead test (D-IBT) in semen were assessed by the peroxidase assay. Results None of these males with hemospermia were found to display significant levels of ASA in the 3 groups. The number of cases who had increased levels of leukocytes in the semen samples of groups A, B and C It was worth noting that the leukocyte levels are increased in semen of males with hemospermia. (15.3%), 6 (75.0%) and 12 (85.7%), respectively.Conclusion Hemospermia is not associated with the production of ASA.