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为了探讨人GM－CSF基因转移表达对慢性乙型肝炎患者细胞免疫功能的影响，运用携带人GM－CSF基因的重组腺病毒转染慢性乙型肝炎患者PBMCs，表明以重组腺病毒为载体的人GM－CSF基因在转染细胞中，可持续表达1．26±0．065～2．09±011ngs·ml-1·24h－1水平26天左右。通过观察慢性乙型肝炎患者PBMCs在GM－CSF基因转架前后及分泌GM－CSF细胞和慢性乙型肝炎患者PBMCs已共同培养前后淋巴细胞增殖反应和CD3、CD4、CD8、CD16、CD19淋巴细胞比率的变化，提示GM－CSF基因转染的淋巴细胞及与分泌GM－CSF细胞共同培养的淋巴细胞增殖反应增强（P＜0．01），CD3、CD4、CD8、CD16、CD19淋巴细胞比率无明显变化（P＞0．05）．小剂量IL－2（25IU／ml）存在时，GM-CSF基因转染及与分泌GM－CSF细胞共同培养的PBMCs中CD3、CD4、CD16淋巴细胞比率升高（P＜0.01），CD8、CD19淋巴细胞比率未见明显变化（P＜0.05）。
In order to investigate the effect of human GM-CSF gene transfer on cellular immune function in patients with chronic hepatitis B, PBMCs were transfected with recombinant adenovirus carrying GM-CSF gene in patients with chronic hepatitis B, indicating that recombinant adenovirus vector GM-CSF gene in transfected cells, sustainable expression of 1.26 ± 0.065 ~ 2.09 ± 011ngs · ml-1 · 24h-1 levels of about 26 days. PBMCs from patients with chronic hepatitis B before and after GM-CSF gene transfer and PBMCs secreting GM-CSF cells and chronic hepatitis B patients have been co-cultured before and after the proliferation of lymphocytes and CD3, CD4, CD8, CD16, CD19 lymphocyte ratio The results showed that the proliferation of lymphocytes transfected with GM-CSF gene and lymphocytes co-cultured with GM-CSF cells were enhanced (P <0.01), and the ratio of CD3, CD4, CD8, CD16 and CD19 lymphocytes was not significant Change (P> 0.05). The percentage of CD3, CD4 and CD16 lymphocytes in PBMCs transfected with GM-CSF gene and GM-CSF cells secreted by low dose of IL-2 (25IU / ml) increased (P <0.01) There was no significant change in lymphocyte ratio (P <0.05).