为观测幽门螺杆菌 (Hp )全菌抗原和粘膜佐剂LT口服免疫Balb/c小鼠后的粘膜免疫应答 ,采用ELISPOT和ELISA法分析免疫小鼠胃粘膜、派伊尔小结 (PP )抗原特异性抗体分泌细胞和小肠粘液唾液sIgA抗体。结果表明抗原免疫组、抗原 +佐剂组胃粘膜、PP抗原特异性sIgA ASC、IgG ASC数量明显增加 ,尤以sIgA ASC为甚 ,并且抗原 +佐剂组明显高于单纯抗原组和对照组 ;小肠粘液唾液特异sIgA水平两免疫组均明显高于对照组 ,提示口服免疫可有效诱导粘膜免疫应答 ,局部特异sIgA在抗Hp感染中具有重要作用 To observe the mucosal immune response of Balb / c mice orally immunized with H. pylori (Hp) and mucosal adjuvant (LT), the gastric mucosa of mice were immunostained with ELISPOT and ELISA. Peyer’s patch antigen (PP) Sex-antibody-secreting cells and small intestinal mucosal salivary sIgA antibodies. The results showed that the number of sIgA ASC and IgG ASC of gastric mucosa and PP antigen in gastric cancer and antigen + adjuvant group was significantly increased, especially sIgA ASC, and antigen + adjuvant group was significantly higher than that of antigen group and control group. Small intestine mucosa specific saliva sIgA level of the two immune groups were significantly higher than the control group, suggesting that oral immunization can effectively induce mucosal immune response, local specific sIgA in anti-Hp infection has an important role