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建立了液相色谱-串联质谱法测定人血浆中的苯达莫司汀(1)和活性代谢产物γ-羟基苯达莫司汀(2),并考察2的同分异构体对测定的影响。使用Synergi Hydro-RP柱,以甲醇∶5mmol/L乙酸铵溶液(含0.1%甲酸)(54∶46)为流动相,流速梯度洗脱。采用电喷雾电离源,多反应监测模式,正离子检测。在人血浆中共检测到4种羟基代谢物(m/z374.1),其中,2的3种同分异构体的峰面积之和不足其1%。人血浆中1和2在3~8 000 ng/ml和1.5~1 000 ng/ml范围内线性关系良好。1、2的日内和日间RSD小于6.0%和8.0%,准确度为96.2%~106.0%和98.6%~103.0%。
A liquid chromatography-tandem mass spectrometry was established for the determination of bendamustine (1) and the active metabolite γ-hydroxybendamustine (2) in human plasma. The effects of isomers of 2 on the determination of influences. A Synergi Hydro-RP column was used with a mobile phase of methanol: 5 mmol / L ammonium acetate solution (containing 0.1% formic acid) (54:46) as the mobile phase with a gradient of flow rate. Electrospray ionization source, multi-reaction monitoring mode, positive ion detection. A total of four hydroxyl metabolites (m / z 374.1) were detected in human plasma, and the sum of the peak areas of three isomers of 2 was less than 1%. The linearity of 1 and 2 in human plasma ranged from 3 to 8 000 ng / ml and from 1.5 to 1 000 ng / ml. 1,2 day and day RSD less than 6.0% and 8.0%, the accuracy of 96.2% ~ 106.0% and 98.6% ~ 103.0%.