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目的探讨微小染色体维持蛋白-5(minichromosome maintenance-5,MCM-5)和细胞增殖抗原标记物(Ki67)在乳腺癌组织中的表达及临床意义。方法2007年1月至2008年4月广西医科大学第五附属医院采用免疫组织化学方法(SP法)对经手术诊断的45例乳腺癌组织中MCM-5和Ki-67基因蛋白进行检测,分析其表达情况。结果MCM-5、Ki-67的表达与临床分期(P<0.05)、淋巴结转移(P<0.01)显著相关,无淋巴结转移组明显低于淋巴结转移组;Ki-67与ER、PR表达无显著相关。结论MCM-5、Ki-67与乳腺癌的发生发展密切相关,可作为判断肿瘤浸润转移、指导治疗和估计预后的参考指标。特别是MCM-5的表达水平可能是判断乳腺癌的术后生存的独立有效指标,而综合应用上述指标可能更有助于判断预后。
Objective To investigate the expression and clinical significance of minichromosome maintenance-5 (MCM-5) and cell proliferation antigen (Ki67) in breast cancer. Methods From January 2007 to April 2008, the immunohistochemical method (SP method) was used to detect and analyze the protein of MCM-5 and Ki-67 in 45 cases of breast cancer tissues diagnosed by operation from the Fifth Affiliated Hospital of Guangxi Medical University. Its expression. Results The expressions of MCM-5 and Ki-67 were significantly correlated with clinical stage (P <0.05), lymph node metastasis (P <0.01), and no significant difference between lymph node metastasis group and lymph node metastasis group Related. Conclusion MCM-5 and Ki-67 are closely related to the occurrence and development of breast cancer, which can be used as a reference index to judge the invasion and metastasis of tumor, guiding the treatment and estimating the prognosis. In particular, the expression level of MCM-5 may be an independent and effective indicator of postoperative survival in breast cancer, and comprehensive application of the above indicators may be more helpful in the prognosis.