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[目的]探讨苯并(a)芘的神经行为毒性及其与CYP1A1基因多态性的关系。[方法]从某钢铁公司焦化厂选择200名健康成年男性焦炉工人为研究对象。采用自行设计的问卷,收集研究对象的一般情况资料以及与本研究相关的基本信息;用WHO推荐的神经行为核心测试组合(NCTB)测试焦炉工神经行为功能。收集研究对象的班后尿和肘静脉血,用高效液相色谱法检测尿中1-羟基芘(1-OhP)的水平;聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析CYP1A1基因3′非编码区MspI位点多态性;等位基因特异性扩增(ASA)方法检测CYP1A1基因第7外显子Ile-Val位点多态性。[结果]MspI位点野生型(m1/m1)、杂合型(m1/m2)、突变型(m2/m2)个体间神经行为功能各指标差异无统计学意义(P>0.05);第7外显子Ile-Val位点野生型(Ile/Ile)、杂合型(Ile/Val)、突变型(Val/Val)个体间上述指标比较,突变型(Val/Val)倒序数字跨度得分低于野生型(Ile/Ile),差异有统计学意义(P<0.05)。[结论]苯并(a)芘可引起接触人群神经行为功能损害,可能与CYP1A1第7外显子Ile-Val位点多态性有关。
[Objective] To investigate the neurobehavioral toxicity of benzo (a) pyrene and its relationship with CYP1A1 gene polymorphism. [Method] A total of 200 healthy adult male coke oven workers were selected as the research object from a coking plant of a steel company. Self-designed questionnaires were used to collect the general information of the subjects and the basic information related to the study. The neural behavioral core test combination (NCTB) recommended by WHO was used to test the neurobehavioral function of coke oven workers. Urine and elbow venous blood samples were collected from the study subjects and the level of 1-OHP in the urine was detected by high performance liquid chromatography (HPLC-MS). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) The MspI polymorphism of CYP1A1 gene in 3 ’non-coding region was analyzed. The polymorphism of Ile-Val in exon 7 of CYP1A1 gene was detected by allele-specific amplification (ASA). [Results] There were no significant differences in the neurobehavioral functions between the MspI loci (m1 / m1), heterozygous (m1 / m2) and mutant (m2 / m2) individuals Compared with the above indexes in Ile-Val, Ile / Val, and Val / Val in exon, In the wild type (Ile / Ile), the difference was statistically significant (P <0.05). [Conclusion] Benzo (a) pyrene can cause impaired neurobehavioral function in the exposed population and may be related to the polymorphism of the Ile-Val site of exon 7 of CYP1A1.