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目的观察骨髓间充质干细胞(MSCs)对大鼠急性一氧化碳(CO)中毒脑损伤的作用并探讨其机制。方法体外培养MSCs并标记,制备MSCs悬液;100~150 mL/kg腹腔注射CO建立动物模型,随后将标记5-溴脱氧尿嘧啶(Brdu)的MSCs经颈内动脉移植入脑损伤的大鼠体内;采用Morris水迷宫实验检测大鼠神经系统功能;采用免疫组化、尼氏染色方法检测海马损伤区细胞变化。结果MSCs在脑内存活并减少神经细胞凋亡,促进神经细胞结构和功能恢复。MSCs移植1、4周后,MSCs组大鼠水迷宫实验显示空间学习能力有所提高,脑组织损伤明显改善(P<0.05)。结论MSCs移植治疗大鼠CO中毒后的脑损伤具有较好的疗效。
Objective To investigate the effect of bone marrow mesenchymal stem cells (MSCs) on brain injury induced by acute carbon monoxide poisoning in rats and its mechanism. Methods MSCs were cultured in vitro and labeled to prepare suspension of MSCs. 100 ~ 150 mL / kg intraperitoneal injection of CO was used to establish animal model. Then MSCs labeled with Brdu were transplanted via internal carotid artery into rats with brain injury In vivo, Morris water maze test was used to detect the function of the nervous system in rats. Immunohistochemistry and Nissl staining were used to detect the cell changes in hippocampal lesion area. Results MSCs survived in the brain and reduced neuronal apoptosis, and promoted the recovery of nerve cell structure and function. After MSCs were transplanted for 1 and 4 weeks, the water maze test in MSCs group showed that spatial learning ability was improved and brain injury significantly improved (P <0.05). Conclusion MSCs transplantation has a good therapeutic effect on brain injury after CO poisoning in rats.