Cloning and structural and expressional characterization of BcpLH gene preferentially expressed in f

来源 :中国科学C辑(英文版) | 被引量 : 0次 | 上传用户:shifujia
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Vegetative growth of Chinese cabbage undergoes the four successive stages which are characterized with the definite types of juvenile,rosette,folding and head leaves.From shoot tips of Chinese cabbage at early folding stage,we constructed a cDNA library and screened the differentially expressed cDNA clones using the cDNAs derived from developing folding leaves and rosette leaves as probes.One complete length of cDNA clone is designated as BcpLH.Computer alignment matched BcpLH to the domains of double-stranded RNA binding (DBRM) and the homologous regions were recognized between BcpLH and human and mouse double-stranded RNA-binding protein TRBP.PCR expression analysis shows that during vegetative growth BcpLH gene was expressed preferentially in folding leaves at folding stage.Transcripts of BcpLH gene were increased when plants were sprayed with IAA.It is deduced that BcpLH gene may be related to initiation of folding leaf and leafy head and induced by auxin in the aspect of transcriptional expression.
其他文献
建筑工程技术管理的作用在激烈的建筑业市场竞争作用越显重要,在努力做好工程施工技术管理工作的基础上,还需要培养技术人才,合理运用管理的职能与科学的办法,使他们相辅相成、协
报道了由微管组成的星体在爪蟾卵细胞提取物中的组装过程、动态变化及其在核膜装配过程中的作用.用精子染色质在爪蟾卵非细胞体系中诱导细胞核重建.在初期阶段, 总是在精子染
随着我国市场经济体制改革的不断深入,相关部门对消防工程项目建设的要求也越来越高,除了要满足消防安全灭火的要求之外,还要对工程的成本进行严格控制。本文首先介绍了控制消防
由于成本管理是直接决定建筑企业的企业利润的核心要素,因此,要想使建筑企业在经济市场中得到长足的发展,规划好建筑企业的成本管理应该是首要解决的问题。本文通过对我国建筑企
项目经理是施工企业法人代表在项目上的全权委托代理人,作为施工管理全面负责的管理者,他担负着相当重要的作用,他不仅是整个施工项目的管理中心,而且是施工项目工程人员的核心;他
我国建筑市场的竞争愈来愈激烈,虽然施工技术不断发展,但是仍旧有许多工程项目存在质量问题,甚至是出现施工安全事故。本文首先分析了当前建筑工程施工项目的管理现状,并结合笔者
Expression of mRNAs for IGF-I,IGF-binding protein-2 (IGFBP-2),and LH receptor (LHR) as well as their regulations during induced follicular atresia was determine
经济发展带动了我国建筑施工行业的发展,尤其是近几年,人们对建筑施工提出了更高的要求。建筑施工管理作为建筑工程中重要的一环,其管理效率的高低直接影响了施工能否顺利进行,严
Two keratin-like proteins of 64 and 55 ku were purified from suspension cells of Daucus carota L.,and their partial amino acid sequences were determined.The hom
Primary hepatocellular carcinoma (HCC) is one of the most common cancers occurring in human,and there is strong epidemiological evidence suggesting that persist