【摘 要】
：
黑曲霉(Aspergillus niger)的粗酶滤液经过硫酸铵沉淀和四种色谱CM-Sephadex C-50离子交换色谱、DEAESephadex A-50离子交换色谱、POROS 20 HQ离子交换色谱和TSK-G3000SW凝胶
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黑曲霉(Aspergillus niger)的粗酶滤液经过硫酸铵沉淀和四种色谱CM-Sephadex C-50离子交换色谱、DEAESephadex A-50离子交换色谱、POROS 20 HQ离子交换色谱和TSK-G3000SW凝胶色谱,从中提纯了一个新的内切β-葡聚糖苷酶.该酶经SDS-PAGE电泳检测分子量为36.0 KD,该酶活力的最适温度为70℃,最适pH值为3.6.纯化后的该酶只能降解羧甲基纤维素,不能降解微晶纤维素,对蔗糖、麦芽糖和纤维素也不起作用.其N-末端部分氨基酸序列为VFEWFGCNECGAEFTxNIPG.
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