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bZIP转录因子广泛参与了植物生长发育以及抗逆应答的调控,属于重要的转录因子家族.为了系统地阐明番茄bZIP转录因子家族成员特征和基因表达模式,利用生物信息学软件和方法,对番茄基因组中的bZIP转录因子进行了系统的分析研究.结果表明:番茄基因组共编码70个bZIP转录因子基因,划分为14个亚族,即A、B、C、D、E、F、G、H、I、J、K、L、S和X.番茄、拟南芥和水稻bZIP基因的系统进化树揭示了进化过程中Group 3和Group 4的成员数目大大增加,进化过程较激烈.氨基酸保守域DOG1和MFMR分别特定存在于12个和6个bZIP成员蛋白序列中.在番茄各器官组织中,干旱、盐、细菌和病毒侵染胁迫条件下,43个番茄bZIP基因不同程度地被诱导表达.以基因表达值上调或下调1.5倍为阈值,部分bZIP基因的表达水平被显著地诱导上调或下调表达.这些基因很可能参与了调控番茄的果实等组织的生长发育,以及逆境胁迫条件下的防御应答反应.通过荧光实时定量PCR,其中7个代表性番茄bZIP基因,被进一步验证了在叶片和果实中的表达水平.本研究鉴定得到的番茄bZIP转录因子基因,可用于今后番茄育种及改良的基因工程研究.
The bZIP transcription factor is widely involved in the regulation of plant growth and development and stress response and belongs to an important family of transcription factors.In order to systematically elucidate the characteristics and gene expression patterns of bZIP transcription factor family members in tomato, bioinformatics software and methods were used to analyze the expression of tomato genome BZIP transcription factor was systematically analyzed and the results showed that: the tomato genome encodes 70 bZIP transcription factor genes, divided into 14 subfamilies, namely A, B, C, D, E, F, G, H, I, J, K, L, S and X. Phylogenetic tree analysis of the bZIP genes in tomato, Arabidopsis and rice revealed that the number of members of Group 3 and Group 4 increased greatly during the evolutionary process, and the evolutionary process was more intense.Amino acid conserved domain DOG1 And MFMR were found in 12 and 6 bZIP members respectively.The bZIP genes of 43 tomato plants were induced to different extents under drought, salt, bacteria and virus infection stress The gene expression was up-regulated or down-regulated by a factor of 1.5, and some of the bZIP gene expression levels were significantly up-regulated or down-regulated.These genes may be involved in the regulation of the growth of tomato and other tissues Development and defensive response under stress conditions.Three representative tomato bZIP genes were further validated in leaf and fruit by real-time fluorescence quantitative PCR.The results showed that the bZIP transcription factor gene , Can be used for future genetic breeding of tomato breeding and genetic engineering.