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目的 探讨癌基因ｒａｓｐ２１、 Ｃ－ｍｙｃ、Ｃ－ｅｒｂＢ－２和ＡＦＰ在２－乙酰氨基芴诱发的实验性大鼠肝癌变过程中的分布情况及关系。方法ｒａｓｐ２１、Ｃ－ｅｒｂＢ－２、Ｃ－ｍｙｃ和ＡＦＰ，采用免疫组织化学ＳＰ方法检测分析。结果 诱癌早期小叶周边增生及变异的肝细胞灶中即显示ｒａｓｐ２１、ＡＦＰ和Ｃ－ｍｙｃ的表达，并随增生肝细胞结节的形成和演进，所表达的细胞也增多并且相伴而存。诱癌中期出现Ｃ－ｅｒｂＢ－２的表达。结论 癌基因蛋白ｒａｓｐ２１、Ｃ－ｍｙｃ、ＡＦＰ是大鼠实验性肝癌变中较早出现的分子改变，可能与肝癌的启动和演进有关，也是肝癌形态发生的分子基础之一。三者具有协同作用。而Ｃ－ｅｒｂＢ－２的异常表达则可能对癌前期病变的发展具有促进作用。同时也表明肝细胞的恶性转化需要多个癌基因的协同作用。
Objective To investigate the distribution and relationship of the oncogenes rasp21, C-myc, C-erbB-2 and AFP in the rat experimental liver cancer induced by 2-acetylamino guanidine. Methods Rasp21, C-erbB-2, C-myc and AFP were detected by immunohistochemical SP method. Results The expressions of rasp21, AFP and C-myc were observed in the hepatic foci around the early stage of the induced lobular hyperplasia. With the formation and evolution of the hepatocyte nodule, the number of expressed cells increased and they were associated with each other. The expression of C-erbB-2 was found in the middle of induction of cancer. Conclusion The oncogene proteins rasp21, C-myc, and AFP are molecular alterations in the early stage of experimental hepatocarcinogenesis in rats, which may be related to the initiation and progression of liver cancer, and are also one of the molecular basis for the morphogenesis of hepatocellular carcinoma. The three have a synergistic effect. The abnormal expression of C-erbB-2 may promote the development of precancerous lesions. It also shows that the malignant transformation of liver cells requires the synergy of multiple oncogenes.