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为了解慢性病毒性肝炎肝组织中转化生长因子β_1(TGFβ_1)及TGFβ_1mRNA的表达情况及其与肝脏病理改变的关系,本文用免疫组织化学法检测了45例慢性病毒性肝炎患者肝脏TGFβ_1表达,并做其中21例TGFβ_1mRNA原位杂交。结果TGFβ_1肝组织表达阳性率为88.89％,主要为间质细胞、汇管区及纤维化发生活跃部位阳性,且随肝纤维化程度加重而表达增强(r=0.73,P<0.05);TGFβ_1mRNA表达阳性率为57.14％,不仅定位于单核细胞、窦细胞,某些肝细胞也有表达。结果表明TGFβ_1与肝纤维化密切相关,不仅间质细胞能够产生TGFβ_1促进肝纤维化,肝细胞本身可能也参与TGFβ_1分泌环路,在肝纤维化发病机制中起一定作用。
In order to understand the expression of transforming growth factor β 1 (TGFβ 1) and TGFβ 1 mRNA in liver tissues of chronic viral hepatitis and its relationship with liver pathological changes, the expression of TGFβ 1 in liver of 45 patients with chronic viral hepatitis was detected by immunohistochemistry 21 cases of TGFβ_1 mRNA in situ hybridization. Results The positive expression rate of TGFβ 1 in liver tissue was 88.89%, which was mainly positive for interstitial cells, portal area and fibrosis. The expression of TGFβ 1 mRNA was increased with the degree of liver fibrosis (r = 0.73, P <0.05) Rate of 57.14%, not only located in monocytes, sinusocytes, some of the liver cells are also expressed. The results showed that TGFβ_1 is closely related to hepatic fibrosis. Not only the interstitial cells can produce TGFβ - 1 to promote hepatic fibrosis, but also the hepatocytes themselves may participate in the secretion of TGFβ - 1, which may play a role in the pathogenesis of hepatic fibrosis.