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目的 探讨妊娠高血压综合征 (妊高征 )患者血管内皮细胞损伤的原因和机理。方法将 10例重度妊高征患者 (妊高征组 )和 10例非妊高征孕妇 (非妊高征组 )的血清分别和人脐静脉内皮细胞共同培养 48h ,同时加入或不加入正常人中性粒细胞 ,观察内皮细胞凋亡的情况和细胞周期的变化。结果 不加入中性粒细胞时 ,两组均无明显的内皮细胞凋亡发生 ;加入中性粒细胞后 ,两组都有凋亡细胞出现 ,妊高征组细胞凋亡的比例明显高于非妊高征组 ;加入或不加入中性粒细胞 ,两组细胞周期都表现为G2 /M期比例明显降低 ,各组间细胞周期变化无明显差异。结论 妊高征患者的血清可能是通过活化中性粒细胞 ,诱导内皮细胞凋亡 ,间接导致内皮细胞的损伤。
Objective To investigate the causes and mechanism of vascular endothelial cell injury in patients with pregnancy induced hypertension (PIH). Methods Serum from 10 patients with severe PIH (PIH group) and 10 non-PIH pregnant women (non-PIH group) were incubated with human umbilical vein endothelial cells for 48h with or without normal control Neutrophils, observed endothelial cell apoptosis and cell cycle changes. Results When neutrophils were not added, no apoptosis of endothelial cells was observed in both groups. After adding neutrophils, apoptotic cells appeared in both groups, and the percentage of apoptosis in PIH group was significantly higher than that in non-neutrophils PIH group; with or without adding neutrophils, the cell cycle of both groups showed G2 / M phase was significantly reduced, the cell cycle between the groups showed no significant difference. Conclusions The serum of patients with PIH may induce endothelial cell apoptosis through activation of neutrophils and indirectly lead to endothelial cell injury.