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目的:探讨白介素-10(IL-10)对缺氧复氧后干扰素调节因子-1(IRF-1)表达的作用。方法:采用缺氧8h复氧2h法建立脑微血管内皮细胞和中性粒细胞共培养缺氧复氧模型。实验分组:正常对照组,缺氧复氧组、IL-10干预组,其中IL-10干预组根据浓度不同分为1μg/L组、10μg/L组和30μg/L组。RT-PCR和Western blot检测IRF-1表达的变化。结果:缺氧复氧组IRF-1表达较正常组显著升高(P<0.05)。IL-10干预组IRF-1表达较缺氧复氧组显著下降,并与IL-10剂量有关,呈剂量依赖性,在30μg/L表达最低(P<0.05)。结论:IL-10可显著抑制脑缺氧复氧后IRF-1的表达。
Objective: To investigate the effect of interleukin-10 (IL-10) on the expression of Interferon Regulator-1 (IRF-1) after hypoxia-reoxygenation. Methods: The hypoxia-reoxygenation model was established by hypoxia for 8 hours and reoxygenation for 2 hours to establish cerebral microvascular endothelial cells and neutrophils co-culture. The experimental group was divided into normal control group, hypoxia-reoxygenation group and IL-10 intervention group. The IL-10 intervention group was divided into 1μg / L group, 10μg / L group and 30μg / L group according to the concentration. The changes of IRF-1 expression were detected by RT-PCR and Western blot. Results: The expression of IRF-1 in hypoxia-reoxygenation group was significantly higher than that in normal group (P <0.05). IL-10 intervention group IRF-1 expression was significantly decreased compared with hypoxia-reoxygenation group, and IL-10 dose-related, in a dose-dependent manner, the lowest expression at 30μg / L (P <0.05). Conclusion: IL-10 can significantly inhibit the expression of IRF-1 after hypoxia-reoxygenation.