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目的:探讨参苓白术散对严重创伤大鼠软组织修复中新生毛细血管与成纤维细胞的影响。方法:对健康成年实验大鼠进行股动脉放血结合刀割法制备严重创伤模型,随机分为活血化瘀组11只、健脾组12只、对照组12只。活血化瘀组给予桃红四物汤治疗,健脾组予参苓白术散治疗,对照组灌服蒸馏水。对3组大鼠术后第6、11、15天的创面进行取材,制作组织切片,采用图像分析系统对切片组织的新生毛细血管与成纤维细胞数量进行阳性率分析。结果:健脾组大鼠在术后第6、11、15天的新生毛细血管以及成纤维细胞数量都优于活血化瘀组,差异有统计学意义(P<0.05);健脾组与活血化瘀组在术后各个阶段的新生毛细血管与成纤维细胞数量都优于对照组,差异有统计学意义(P<0.05)。结论:参苓白术散对严重创伤大鼠软组织修复中新生毛细血管与成纤维细胞的增加有着明显的作用,能够促进软骨组织的有效修复。
Objective: To investigate the effect of Shenling Baizhu San on neovascularization and fibroblasts repair in soft tissue in severely traumatized rats. Methods: A severe traumatic model was established in healthy adult rats by means of femoral artery bleeding combined with knife-cutting method. The models were randomly divided into 11 blood-activating and blood-stasis-removing groups, 12 spleen-qi group and 12 control group. Huoxuehuayu group given Taohong Siwu Decoction treatment, spleen group to Shenling Baizhu San treatment, the control group irrigated with distilled water. The wounds on the 6th, the 11th and the 15th postoperative days were harvested from the three groups of rats. Tissue sections were made. The positive rate of the number of newborn capillaries and fibroblasts in the sections was analyzed by image analysis system. Results: The number of newborn capillaries and fibroblasts in the spleen group were better than those in activating blood circulation and removing blood stasis group on the 6th, 11th and 15th day after operation (P <0.05) The number of newborn capillaries and fibroblasts in all stages of Huayu group were better than those in control group, the difference was statistically significant (P <0.05). Conclusion: Shenling Baizhu Powder has a significant effect on the increase of capillaries and fibroblasts in soft tissue repair of severely traumatic rats, which can promote the effective repair of cartilage tissue.