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目的有效分离甲型流感病毒,提高病毒分离率。方法选择在2012年10月-2014月5月期间,经实时定量荧光PCR(real-time PCR)检测为甲型流感病毒阳性的咽拭样品,并采用MDCK细胞进行病毒分离;对血凝素(HA)滴度<8的一代培养物分别进行原倍、2倍、5倍、10倍稀释后进行传代培养,比较不同稀释度传代培养物血凝实验效果。结果 2倍稀释培养物传代培养后,血凝实验效果分别与其他3种进行比较,差异有统计学意义。结论甲型流感病毒分离中,对培养物进行2倍稀释后病毒分离效果最好。
Objective Isolation of influenza A virus, improve the virus isolation rate. Methods The pharyngeal swab samples positive for influenza A virus were detected by real-time PCR during the period from October 2012 to May 2014 and virus isolation was performed using MDCK cells. The hemagglutinin HA) titers <8 were subcultured at the original, 2, 5, and 10-fold dilutions, respectively. The effects of different dilutions of subcultures on blood coagulation were compared. Results The results of two-fold diluted culture subculture, the blood coagulation test results were compared with the other three, the difference was statistically significant. Conclusions In the isolation of influenza A virus, the virus is best separated after two times dilution of the culture.