论文部分内容阅读
目的:研究高表达缺氧诱导因子(HIF-1α)对肾癌(GRC-1)细胞增殖和细胞内斯钙素-1(STC-1)、Ca2+水平的影响。方法:构建HIF-1α重组质粒,转染GRC-1细胞,MTT法检测GRC-1细胞的增殖,RT-PCR法检测GRC-1细胞内HIF-1α及STC-1的表达,荧光探针检测细胞内Ca2+水平,同时设正常GRC-1细胞作为对照组。结果:与对照组相比,转染了HIF-1α的GRC-1细胞增殖率和STC-1表达水平显著增高(P<0.05),Ca2+水平明显降低(P<0.05)。结论:HIF-1α可能参与了肾癌细胞的恶性增殖,其机制可能与STC-1下调肿瘤细胞内Ca2+水平有关。
OBJECTIVE: To investigate the effect of HIF-1α on the proliferation of human renal carcinoma (GRC-1) cells and on the levels of staurosporine-1 (STC-1) and Ca2 +. METHODS: HIF-1α recombinant plasmid was constructed and transfected into GRC-1 cells. The proliferation of GRC-1 cells was detected by MTT assay. The expression of HIF-1α and STC-1 in GRC-1 cells was detected by RT- Intracellular Ca2 + levels, while setting normal GRC-1 cells as a control group. Results: Compared with the control group, the proliferation rate of GRC-1 cells transfected with HIF-1α and the expression of STC-1 were significantly increased (P <0.05) and the levels of Ca2 + were significantly decreased (P <0.05). Conclusion: HIF-1α may be involved in the malignant proliferation of renal cell carcinoma. The mechanism may be related to the downregulation of intracellular Ca2 + by STC-1.