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目的 研究氢化可的松 (HC)对大鼠学习记忆功能的影响及与氧自由基的关系。方法 迷宫法检测实验大鼠的学习记忆功能 ;Lowry法及硫代巴比妥酸法分别测定胞浆及线粒体的蛋白质及MDA含量 ;分光光度法及荧光分光光度法分别测定胞浆及线粒体的GSH及GSSG的含量 ;黄嘌呤氧化酶法及紫外分光光度法分别测定胞浆及线粒体的Mn SOD及CAT的含量。结果 HC可引起老龄前期大鼠记忆障碍 ,同时使脑细胞浆与线粒体产生MDA及GSSG增加、GSH及GSH/GSSG比值下降以及Mn SOD及CAT活性降低。结论 HC同步抑制老龄前期大鼠学习记忆功能是与其降低机体的抗氧化能力和增加氧自由基的损伤有关
Objective To study the effect of hydrocortisone (HC) on learning and memory in rats and its relationship with oxygen free radicals. Methods The maze test was used to test the learning and memory function of rats. The contents of MDA and protein in cytoplasm and mitochondria were measured by Lowry and thiobarbituric acid methods, respectively. The contents of GSH in cytoplasm and mitochondria were measured by spectrophotometry and fluorescence spectrophotometry And GSSG contents. The contents of Mn SOD and CAT in cytoplasm and mitochondria were determined by xanthine oxidase and UV spectrophotometry respectively. Results HC can cause memory impairment in presenile rats, increase MDA and GSSG, reduce the ratio of GSH and GSH / GSSG, and decrease the activity of Mn SOD and CAT in brain mitochondria. Conclusions HC inhibition of learning and memory in presenile rats is associated with its ability to reduce the body’s antioxidant capacity and increase oxygen free radical damage