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采用免疫标记法,使用流式细胞仪测定受到2.5Gy6MV-X射线照射的脐带血AC133+细胞加细胞因子组合(IL-3+FL+SCF)在培养不同天数后的细胞周期及早期凋亡情况的变化。结果表明,新鲜分离的未受照的脐带血AC133+细胞在无细胞因子的短期液体培养体系中培养2天,99%的细胞处于G0/G1期;受照的脐带血AC133+细胞加细胞因子组合(IL-3+FL+SCF)培养不同天数后,脐带血AC133+细胞迅速进入增殖循环,在照后第3天,有近50%的细胞进入S期。脐带血AC133+细胞受照后不加细胞因子,培养48小时后,镜下观察到细胞全部死亡;加细胞因子组合(IL-3+FL+SCF)培养48小时后,有(38.0±6.8)%的受照的脐带血AC133+细胞被“救活”,且随着培养时间的延长,被“救活”的AC133+细胞又出现了增殖。因此,可认为细胞因子组合(IL-3+FL+SCF)对受照的脐带血AC133+细胞具有保护作用。
Immunocytochemistry was used to determine the cell cycle and early apoptosis of cord blood AC133 + cells and cytokines (IL-3 + FL + SCF) exposed to 2.5Gy6MV-X-rays after culturing for different days by flow cytometry Variety. The results showed that freshly isolated UC133 + untreated umbilical cord blood cells were cultured in a cytokine-free short-term liquid culture system for 2 days with 99% of cells in the G0 / G1 phase; irradiated umbilical cord blood AC133 + cells plus cytokines ( IL-3 + FL + SCF) cultured for different days, umbilical cord blood AC133 + cells rapidly into the proliferation cycle, on the 3rd day after irradiation, nearly 50% of cells into the S phase. Umbilical cord blood AC133 + cells without cytokines after irradiation, 48 hours after culture, the cells were observed under the microscope all died; plus cytokines combination (IL-3 + FL + SCF) after 48 hours of training, (38.0 ± 6.8)% Of irradiated umbilical cord blood AC133 + cells were “rescued” and the “rescued” AC133 + cells proliferated as the incubation time extended. Therefore, the combination of cytokines (IL-3 + FL + SCF) can be considered to have a protective effect on irradiated cord blood AC133 + cells.