论文部分内容阅读
目的:观察紫金胶囊抗乙型肝炎病毒的作用。方法:分别采用20mg/ml、10mg/ml、5mg/ml、2.5mg/ml的药液,在乙型肝炎病毒基因转染的人肝癌细胞系2,2,15细胞培养中,观察其对细胞的毒性和对HBeAg和HBsAg分泌的抑制效果。结果:紫金胶囊加入细胞培养液中培养12d,对细胞的半数有毒浓度为20mg/ml,最大无毒浓度为10mg/ml。最大无毒浓度10mg/ml时抑制细胞分泌HBsAg为66.3%0.76%(P<0.001),半数抑制浓度为(6.40.66)mg/ml,治疗指数为3.150.34;5mg/ml时HBsAg cpm值与细胞对照组比较有显著意义(P<0.05);10mg/ml抑制细胞分泌HBeAg为43.4%1.28%(P<0.001),半数抑制浓度为(7.640.24)mg/ml,治疗指数为2.620.08;2.5mg/ml时HBeAg cpm值与细胞对照组比较,仍有显著性差异(P<0.05)。结论:紫金胶囊在2,2,15细胞中培养12d,无毒浓度可明显抑制HBsAg和HBeAg的分泌。
Objective: To observe the anti-hepatitis B virus effect of Zijin Capsule. METHODS: 20 mg/ml, 10 mg/ml, 5 mg/ml, and 2.5 mg/ml drug solutions were used to observe the effect on the cells of human hepatocellular carcinoma cell lines 2, 2 and 15 transfected with the hepatitis B virus gene. The toxicity and inhibition of HBeAg and HBsAg secretion. RESULTS: Zijin capsules were cultured in cell culture medium for 12 days. The half toxic concentration of the cells was 20 mg/ml, and the maximum non-toxic concentration was 10 mg/ml. The maximum nontoxic concentration of 10 mg/ml inhibited the secretion of HBsAg by 66.3% and 0.76% (P<0.001), the half inhibition concentration was (6.40.66) mg/ml, the therapeutic index was 3.150.34, and the HBsAg cpm value was 5 mg/ml. Compared with the cell control group, there was significant difference (P<0.05); 10mg/ml inhibited HBeAg secretion by 43.4% and 1.28% (P<0.001), half inhibition concentration was (7.640.24) mg/ml, and the therapeutic index was 2.620. The value of HBeAg cpm at 08;2.5mg/ml was still significantly different from the control group (P<0.05). Conclusion: Zijin Capsules were cultured in 2,2,15 cells for 12 days. The non-toxic concentration can significantly inhibit the secretion of HBsAg and HBeAg.