论文部分内容阅读
目的:建立HPLC测定急性子中总黄酮含量的方法。方法:采用Eclipse XDB-C18柱(4.6 mm×150 mm,5μm);流动相甲醇-0.4%磷酸水(55∶45),检测波长365 nm,柱温30℃,流速1.0 mL.min-1。结果:槲皮素、山柰酚、异鼠李素的线性范围分别为0.111 2～0.556 0(r=0.999 4),0.007 711～0.023 23(r=0.999 4),0.006 69～0.033 45μg(r=0.999 2);平均加样回收率分别为98.5%(RSD 1.3%,n=6),97.3%(RSD 1.7%,n=6),97.6%(RSD 1.6%,n=6)。结论:该方法准确度高,重复性好,可用于急性子中总黄酮的含量测定。
Objective: To establish a HPLC method for the determination of total flavonoids in acute subtilis. Methods: Eclipse XDB-C18 column (4.6 mm × 150 mm, 5 μm) was used. The mobile phase consisted of methanol and 0.4% phosphoric acid water (55:45). The detection wavelength was 365 nm and the column temperature was 30 ℃. Results: The linear ranges of quercetin, kaempferol and isorhamnetin were 0.111 2 ~ 0.556 0 (r = 0.999 4), 0.007 711 ~ 0.023 23 (r = 0.999 4), 0.006 69 ~ 0.033 45 μg = 0.999 2). The average recoveries were 98.5% (RSD 1.3%, n = 6), 97.3% (RSD 1.7%, n = 6) and 97.6% (RSD 1.6%, n = 6). Conclusion: The method is accurate, reproducible and can be used to determine the content of total flavonoids in acute.