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目的比较腭扁桃体细胞 (PTC)与外周血单个核细胞 (PBMC)的表型 ,观察IL 2刺激前后PTC的杀伤活性,探讨扁桃体的免疫功能。方法用流式细胞仪分别检测扁桃体与PBMC表面CD3,CD4 ,CD8 ,CD20,PTA1及9.1C3的表达情况。用4h51Cr释放实验检测静止时及IL 2刺激后PTC中NK细胞杀伤K562活性的改变。结果PTC中CD20的表达率为71.2 % ,PBMC为15.5 % ,且PTC中的平均荧光强度 (MFI)明显高于PBMC。静止的扁桃体细胞杀伤功能非常低 ,经IL 2刺激后杀伤功能显著提高 ,效靶比为200∶1时达到61.5 %。CD3阳性率在PTC和PBMC中分别为32.8%和57.7% ;CD4/CD8的比值分别为6.97和1.11。两种新分子血小板T细胞活化抗原1(PTA1)和9.1C3在扁桃体中均有表达。结论扁桃体单个核细胞中B细胞占多数 ,而且CD20的表达密度明显高于外周血B细胞 ;在T细胞中以CD4 +细胞为主 ;IL 2活化后PTC有较高的自然杀伤活性 ;与CTL和NK细胞相关的膜标记PTA1和9.1C3均有表达。
Objective To compare the phenotypes of palatine tonsil cells (PTC) and peripheral blood mononuclear cells (PBMCs), observe the cytotoxicity of PTC before and after IL 2 stimulation, and explore the immune function of tonsils. Methods The expressions of CD3, CD4, CD8, CD20, PTA1 and 9.1C3 on tonsil and PBMC were detected by flow cytometry. 4h51Cr release test was used to measure the change of NK cell killing K562 activity in resting and IL2-stimulated PTC. Results The positive rate of CD20 expression in PTC was 71.2% and PBMC was 15.5%, and the mean fluorescence intensity (MFI) in PTC was significantly higher than that in PBMC. Quiescent tonsil cell killing function is very low, the killing function after IL 2 stimulation significantly increased, the effective target ratio of 200: 1 reached 61.5%. The positive rates of CD3 were 32.8% and 57.7% in PTC and PBMC, respectively. The ratios of CD4 / CD8 were 6.97 and 1.11, respectively. Two new molecules, T-cell activating antigen 1 (PTA1) and 9.1C3, are expressed in the tonsils. Conclusion B cells in tonsillar mononuclear cells account for the majority, and the expression density of CD20 is significantly higher than that of peripheral blood B cells. CD4 + cells are predominant in T cells; PTCs have higher natural killer activity after IL2 activation; Membrane markers associated with NK cells, PTA1 and 9.1C3, were expressed.