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采用高效液相色谱梯度法分离测定雷公藤制剂中雷公藤甲素的含量，用国产YWG色谱柱分离，二极管阵列检测器检测，所得三维光谱与空白光谱进行差减，取波长218nm处色谱图积分，定量。与等度法比较，梯度法具有更高的分离度、灵敏度，测定雷公藤口服液中雷公藤甲素的质量浓度为202．0μg／L，每片雷公藤片剂含甲素11．74μg，结果显著低于等度法和标示量（P<0．05）。
The content of triptolide in Tripterygium wilfordii was determined by high performance liquid chromatography (HPLC) gradient method, separated by domestic YWG column and detected by diode array detector. The difference between the obtained three-dimensional spectrum and the blank spectrum was subtracted, , Quantitative. Compared with the isocratic method, the gradient method has a higher resolution and sensitivity. The content of triptolide in the triptolide oral solution was 202.0μg / L, and the tablet contained 11.74μg of A, Results were significantly lower than the isometric method and labeled amount (P <0.05).