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应用巢式 -PCR方法对 3例HIV - 1感染者外周血单个核细胞中的HIV - 1膜蛋白基因进行扩增 ,并对C2 -V3及其邻区 36 0个核苷酸序列进行测定和分析 ,计算基因离散率和系统进化树。结果 ,3例感染者体内有B和C或B′和E两种不同HIV - 1亚型毒株序列 ,各亚型毒株与相应的Bcon、Ccon、Econ国际亚型毒株序的基因离散率分别为 5 0 7～ 7 2 6 %、3 2 3%、5 38～ 5 99% ;同一个体内不同亚型毒株序列间的基因离散率分别为 2 3 5 2 %、2 0 6 9%、2 8 44 % ;根据gp12 0V3环顶端四肽序列特征 ,发现有卖淫和静脉吸毒史妓女体内的sz46- 1具有典型的欧美B亚型GPGR序列 ,sz8- 2为GPGH ,其它毒株均为泰国B(B′)和E亚型所特有的GPGQ序列 ;进一步系统树分析显示 ,sz1- 2、sz8- 1、sz46 - 1与Bcon丛集一起 ,sz1- 1、sz8- 2和sz46 - 1分别与Econ、Ccon丛集。结论 :同一个体可以同时感染不同亚型HIV - 1毒株 ,多种高危行为可能是导致混合感染的主要原因
The HIV-1 membrane protein gene in peripheral blood mononuclear cells from 3 HIV-1 infected patients was amplified by nested-PCR and the nucleotide sequence of 36 0 nucleotides in C2-V3 and its adjacent regions was determined. Analyze and calculate genetic divergence rate and phylogenetic tree. As a result, there were two different strains of HIV - 1 subtype B and C or B ’and E in the three infected individuals, and the genes of each subtype and the corresponding Bcon, Ccon and Econ isolates The rates of gene dispersal among different subtypes of the same in vivo were respectively 25.32%, 26.03%, 35.39% and 50.6%, respectively %, 2 8 44%. According to the tetrapeptide sequence of gp12 0V3, it was found that sz46-1 in typical prostitutes and drug users had GPGR sequences of European and American subtype B, sz8-2 was GPGH, Further phylogenetic tree analysis showed that sz1-2, sz8-1, sz46-1, together with the Bcon cluster, sz1-1, sz8-2, and sz46-1, are specific for the B (B ’) and E subtypes in Thailand Respectively with the Econ, Ccon cluster. Conclusion: The same individual can infect different HIV - 1 strains at the same time. A number of high risk behaviors may be the main reason of mixed infection