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研究在培养液中添加亚硫酸和低温处理培养液的效应,从而以简化离体小麦穗的培养。应用简化方法,可在含100g/l蔗糖和0.075％亚硫酸的液体培养基上,不经过任何消毒,室温下培养离体穗。激动索和胰蛋白酶虽可降低光周期反应和狭义早熟性,但化学物品处理不影响未成熟胚的低温要求。低温处理显著影响低温要求。开花后10天的离体穗经低温处理,可获得具有正常萌发力的完全春化的成熟种子。本试验旨在研究在培养液中添加亚硫酸液(Yasuda,1948)和冷处理培养液(Donovan和Lee,1977)的效应。采用这种方法,用低温或化学物品处理未成熟种子,并测定处理对抽穗的影响。
The effects of adding sulphurous acid in the broth to the broth at low temperature were studied to simplify the cultivation of isolated wheat spikes. Using a simplified method, ex vivo spikes can be cultivated on liquid media containing 100 g / l sucrose and 0.075% sulfurous acid without any sterilization. Although both agouti and trypsin can reduce the photoperiodic response and the narrow premature, chemical treatment does not affect the cryogenic requirements of immature embryos. Cryogenic treatment significantly affects the cryogenic requirements. In vitro panicles at 10 days after flowering are treated with low temperature to obtain fully vernalized mature seeds with normal vigor. The aim of this study was to investigate the effect of adding sulfite solution (Yasuda, 1948) and cold-treated broth (Donovan and Lee, 1977) to broth. Using this method, immature seeds were treated with cryogenic chemicals or chemicals and the effect of treatment on heading was determined.