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过碘酸-雪夫氏(PAS)反应物质包括糖原,粘多糖,粘蛋白和糖蛋白,糖脂及少数不饱和脂类及磷脂类。骨髓巨核细胞糖原在血液病的改变报道较少,我们对61例的骨髓巨核细胞糖原进行了初步观察。材料和方法一、病例正常对照10名,无贫血或出血,无肝脾肿大,血小板计数、骨髓象及巨核细胞形态均属正常。51例疾病中主要为贫血、紫瘢、脾功能亢进、急性感染及少数其他血液病。临床诊断根据症状、血液学及其他实验室检查,少数病例经手术或病理检查证实。二、PAS染色新鲜骨髓涂片在空气中干燥后以95%乙醇固定5分钟,瑞氏液染
Periodic acid-Schiff’s (PAS) reactive substances include glycogen, mucopolysaccharides, mucins and glycoproteins, glycolipids, and few unsaturated lipids and phospholipids. Marrow megakaryocytic glycogen in blood disease changes reported less, we have 61 cases of bone marrow megakaryoblast glycogen were initially observed. Materials and methods First, the case of normal control 10, no anemia or bleeding, no hepatosplenomegaly, platelet count, bone marrow and megakaryocyte morphology are normal. 51 cases of disease mainly anemia, purple scar, hypersplenism, acute infection and a few other blood diseases. Clinical diagnosis based on symptoms, hematology and other laboratory tests, a small number of cases confirmed by surgery or pathological examination. Second, PAS staining fresh bone marrow smear in the air after drying with 95% ethanol for 5 minutes, Wright liquid dyed