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本研究探讨S 2 (3 氨丙基氨基 )乙基硫代磷酸酯 (WR 2 72 1,Amifostine)对白血病细胞系HL 6 0细胞增殖与凋亡的影响。采用XTT法检测细胞增殖及药物敏感性 ,用annexin/PI双染色法检测细胞凋亡 ,用流式细胞术检测细胞周期。结果表明 :WR 2 72 1对HL 6 0细胞增殖呈浓度时间依赖性抑制作用 ,0 .5mmol/LWR 2 72 137℃处理 30分钟后的HL 6 0细胞对VP16的敏感性增强 ,IC50 由 5 2 .5 μg/ml下降为 4 0 .5 μg/ml。WR 2 72 15 .0mmol/L作用 72小时后 ,早期细胞凋亡由 (5 .5± 1.9) %增加至 (4 8.5± 8.4 ) % (P <0 .0 0 1) ,晚期细胞凋亡由 (1.2± 0 .5 ) %增加至 (39.0± 4 .0 ) % (P <0 .0 0 1) ,并出现G2 M期细胞阻滞。结论 :S 2 (3 氨丙基氨基 )乙基硫代磷酸酯能增强HL 6 0细胞对VP16的敏感性 ,并可通过G2 M期阻滞 ,诱导细胞凋亡 ,从而抑制HL 6 0细胞的增殖。
This study was aimed to investigate the effects of S 2 (3 aminopropylamino) ethyl phosphorothioate (WR 2 72 1, Amifostine) on the proliferation and apoptosis of leukemia cell line HL 60. Cell proliferation and drug sensitivity were detected by XTT assay. Apoptosis was detected by annexin / PI double staining and cell cycle was detected by flow cytometry. The results showed that WR 2 72 1 had a time-dependent inhibitory effect on the proliferation of HL-60 cells. The sensitivity of VP-6 cells to HL-60 after 30 minutes treatment with 0.5 mmol / L WR 2 72 at 137 ° C was enhanced, and the IC50 increased from 52 .5 μg / ml decreased to 40.5 μg / ml. The apoptosis of early stage cells increased from (5.5 ± 1.9)% to (4. 8.5 ± 8.4)% (P <0.01) after 72 hours of treatment with WR 2 72. (1.2 ± 0.5%) increased to (39.0 ± 4.0)% (P <0.01), and G2 arrest appeared. CONCLUSION: S 2 (3-aminopropylamino) ethyl phosphorothioate enhances the sensitivity of HL-60 cells to VP16 and induces cell apoptosis via G2-M arrest and thus inhibits HL-60 cell proliferation.