论文部分内容阅读
目的研究积雪草有效成分积雪草苷的抗纤维化作用。方法建立猪血清致大鼠免疫性肝纤维化模型,设正常组、模型组、γ干扰素组、积雪草苷高、中、低剂量组(0.9,0.3,0.1 g·kg~(-1)),检测大鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)以及肝组织中羟脯氨酸(Hyp)的含量,HE及Masson染色观察肝组织病理学变化,并且对纤维化程度进行半定量评分。结果积雪草苷能显著降低大鼠血清中的ALT、AST水平以及肝组织中的Hyp水平;积雪草苷各剂量组均能减轻大鼠肝组织的纤维化程度,其中高剂量组尤为显著(P<0.01)。结论积雪草苷具有抗免疫性大鼠的肝纤维化作用,且呈现出一定的量效关系。
Objective To study the anti-fibrosis effect of asiaticoside, the active ingredient of Centella asiatica. Methods The model of immunological liver fibrosis induced by porcine serum was established. The normal group, model group, interferon gamma group and asiaticoside high, middle and low dose groups (0.9, 0.3, 0.1 g · kg -1 ), The content of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and hydroxyproline (Hyp) in liver tissue of rats were detected. The pathological changes of liver tissue were observed by HE and Masson staining, and the degree of fibrosis Semi-quantitative score. Results Asiaticoside could significantly decrease the levels of ALT and AST in serum and Hyp levels in liver tissue. Asiaticoside in each dose group could reduce the degree of liver fibrosis in rats, especially in high-dose group (P <0.01). Conclusion Asiaticoside has anti-immune effects of hepatic fibrosis in rats, and shows a certain dose-effect relationship.