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目的:探讨子痫前期三维能量多普勒超声(3D-PDU)和产后胎盘中MMP-2、TIMP-2蛋白含量及蛋白阳性表达强度的关系。方法:选取在医院产科就诊的门诊及住院孕妇60例,其中正常晚期妊娠20例为对照组,轻度和重度子痫前期患者各20例作为实验组。对所有孕妇胎盘床血管进行3D-PDU,通过VOCAL软件测量血管化指数(VI)、血流指数(FI)和血管化血流指数(VFI)。生产后留取胎盘组织,采用酶联免疫法和免疫组织化学法检测各组孕妇胎盘中MMP-2、TIMP-2蛋白含量及蛋白阳性表达强度。结果:与对照组比较,轻度和重度子痫前期患者的VI、FI、VFI依次降低,差异均有统计学意义(P<0.01);两组患者胎盘组织中MMP-2蛋白含量及蛋白阳性表达强度依次降低,差异均有统计学意义(P<0.01),而TIMP-2的蛋白含量及蛋白阳性表达强度差异无统计学意义(P>0.05)。结论:3D-PDU检测各指标的变化趋势与胎盘组织中MMP-2表达相一致,MMP-2的下调导致MMP-2与TIMP-2的比例失衡,这为子痫前期的诊断及治疗提供新的靶点。
Objective: To explore the relationship between the content of 3D-PDU and the expression of MMP-2, TIMP-2 in postpartum placenta and the expression of protein in preeclampsia. Methods: Sixty pregnant and hospitalized pregnant women were selected from the obstetrics department of the hospital. Among them, 20 cases of normal late pregnancy as control group and 20 cases of mild and severe preeclampsia as experimental group. 3D-PDU was performed on all placenta bed vessels of all pregnant women. The vascularization index (VI), blood flow index (FI) and vascularized blood flow index (VFI) were measured by VOCAL software. The placenta tissues were collected after production, and the protein and expression of MMP-2 and TIMP-2 in the placenta of pregnant women were detected by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry. Results: Compared with the control group, the levels of VI, FI and VFI in the mild and severe preeclampsia patients were significantly lower than those in the control group (P <0.01). The levels of MMP-2 protein and protein in the placenta The expressions of TIMP-2 and TIMP-2 protein were significantly lower than those of the control group (P <0.01). However, there was no significant difference in the protein content and protein expression of TIMP-2 (P> 0.05). Conclusions: The trend of 3D-PDU detection is consistent with the expression of MMP-2 in placental tissue. The down-regulation of MMP-2 results in the imbalance of MMP-2 and TIMP-2, which provides new diagnosis and treatment for preeclampsia The target.