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采用微生物管碟法和比浊法测定注射用硫酸链霉素含量,微生物管碟法是将1.2和0.6 U/mL的抗生素溶液加入到牛津杯中,通过溶液的渗透与摊布在固体平板中的枯草芽孢杆菌相互作用形成抑菌圈,通过测量标准品与供试品形成的抑菌圈直径来计算供试品的含量;比浊法是在石英比色管中按拉丁方排列顺序加入7.5和5.0 U/mL的抗生素溶液及含金黄色葡萄球菌的液体培养基,通过在线测定标准品与供试品吸光度来计算供试品的含量,测定数据表明:微生物管碟法与比浊法测定注射用硫酸链霉素含量结果无显著差异,同时阐述2种试验方法各自的优缺点及应注意的问题。
Microbial plate method and nephelometry were used to determine streptomycin sulfate for injection. Microbial plate method was to add 1.2 and 0.6 U / mL of antibiotic solution to the Oxford cup. The solution was infiltrated and spread on a solid plate Of Bacillus subtilis interaction inhibition zone formed by measuring the diameter of the inhibition zone formed by the standard and the test sample to calculate the amount of the test article; turbidimetric method is in the quartz tube according to the order of the order of Latin 7.5 And 5.0 U / mL of antibiotic solution and Staphylococcus aureus containing liquid medium by measuring the standard and the absorbance of the test sample to calculate the content of the test sample, the measured data show that: Microbial plate and turbidimetric assay There was no significant difference in the streptomycin sulfate content for injection, and the advantages and disadvantages of each of the two methods were also discussed.