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目的:探讨含不同神经肽的神经纤维在碘乙酰胺诱导的大鼠胃炎中的变化,分析神经纤维与免疫细胞的相互关系。方法:采用碘乙酰胺诱导制备大鼠胃炎模型,以纯净水喂养为对照组,采用免疫组织化学法、免疫细胞化学法定量分析对照组及模型组大鼠胃黏膜中含P物质(SP)、神经肽Y(NPY)及血管活性肠肽(VIP)的神经纤维数量及免疫活性细胞。结果:光学显微镜观察模型组和对照组胃黏膜含SP、NPY及VIP神经纤维分布于黏膜层腺体之间。模型组黏膜含SP及VIP的神经纤维数量较对照组明显增加(P<0.05)。模型组大鼠的胃黏膜可见免疫活性细胞,主要为淋巴细胞、浆细胞和肥大细胞,表明SP、NPY表现出免疫活性,免疫细胞与神经纤维毗邻。结论:胃炎模型制作成功;神经肽参与了胃炎的形成过程及免疫调节。
OBJECTIVE: To investigate the changes of nerve fibers containing different neuropeptides in iodoacetamide-induced gastritis in rats, and to analyze the relationship between nerve fibers and immune cells. Methods: The rat model of gastritis was induced by iodoacetamide, and the pure water was used as the control group. Immunohistochemistry and immunocytochemistry were used to quantitatively analyze the content of substance P (SP) in the gastric mucosa of the control group and the model group. Number of nerve fibers and immunocompetent cells of neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP). Results: The gastric mucosa with SP, NPY and VIP in the model group and the control group were distributed between mucosal glands. The number of nerve fibers in the mucosa containing SP and VIP in the model group was significantly higher than that in the control group (P <0.05). The immunocompetent cells, mainly lymphocytes, plasma cells and mast cells, were observed in the gastric mucosa of the model group, indicating that SP and NPY were immunocompetent and the immune cells were adjacent to the nerve fibers. Conclusion: Gastritis model was successfully made. Neuropeptide was involved in the formation of gastritis and immune regulation.