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以地高辛-人血清白蛋白偶联物为抗原,应用B细胞杂交瘤技术融合并筛选出7株分泌抗地高辛单克隆抗体的杂交瘤。该抗体可与地高辛人血清白蛋白特异性结合而不和其它具有类似化学结构的半抗原结合。用抗地高辛单克隆抗体治疗实验性地高辛中毒动物模型,可使地高辛中毒表现迅速消失。作者认为,抗地高辛单克隆抗体可作为地高辛的拮抗剂,对治疗地高辛中毒具有重要意义。
Using digoxigenin-human serum albumin conjugate as antigen, seven hybridomas secreting anti-digoxin monoclonal antibody were fused by using B cell hybridoma technique. The antibody binds specifically to digoxigenin serum albumin without binding to other haptens having similar chemical structures. Anti-digoxin monoclonal antibody treatment of experimental animal models of digoxin poisoning, digoxin poisoning can rapidly disappear. The authors believe that anti-digoxin monoclonal antibody can be used as a digoxin antagonist, the treatment of digoxin poisoning is of great significance.