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目的研究黄连素对结肠上皮隐窝细胞基底膜钙依赖钾通道[I_K(Ca)]和环磷酸腺苷(cAMP)依赖钾通道[I_K(cAMP)]的影响,探讨其治疗分泌性腹泻的机制。方法用乙二胺四乙酸(EDTA)溶液分离结肠上皮隐窝细胞,运用EPC 10膜片钳放大器测量全细胞模式下50、100、500μmol/L黄连素对结肠上皮细胞基底膜I_K(Ca)和I_K(cAMP)的影响,并设PSS对照组。结果50、100、500μmol/L黄连素可抑制大鼠结肠上皮隐窝细胞基底膜I_K(Ca)和I_K(cAMP)(P值均<0.05),当阶跃刺激为+80mV时,其I_K(Ca)分别为对照组的(71.43±3.61)%、(54.56±5.13)%、(38.66±3.85)%(P<0.05);其I_K(cAMP)分别为对照组的(78.55±5.72)%、(60.42±6.33)%、(43.78±6.47)%(P<0.05)。结论黄连素能抑制大鼠结肠上皮细胞基底膜I_K(Ca)和I_K(cAMP)的开放,这可能是其治疗分泌性腹泻的机制之一。
Objective To study the effects of berberine on calcium-dependent potassium channels [I_K(Ca)] and cAMP (cAMP)-dependent potassium channels [I_K(cAMP)] in colonic epithelial crypt cells, and to explore the mechanism of treatment of secretory diarrhea. . Methods The epithelial crypt cells were isolated with ethylenediaminetetraacetic acid (EDTA) solution. The basal membrane I_K(Ca) of colonic epithelial cells was measured with 50, 100, and 500 μmol/L berberine in the whole cell model using EPC 10 patch clamp amplifier. The effect of I_K (cAMP) and set up the PSS control group. Results 50, 100, and 500 μmol/L berberine inhibited the basal membrane I_K (Ca) and I_K (cAMP) in colonic epithelial crypt cells of rats (P<0.05). When step stimulation was +80mV, I_K ( Ca) were (71.43±3.61)%, (54.56±5.13)%, (38.66±3.85)% (P<0.05) in the control group, and I_K (cAMP) were (78.55±5.72)% in the control group, respectively. (60.42±6.33)%, (43.78±6.47)% (P<0.05). Conclusion Berberine can inhibit the opening of I_K (Ca) and I_K (cAMP) in rat colonic epithelial cells, which may be one of the mechanisms of its treatment of secretory diarrhea.