论文部分内容阅读
目的 通过观察矽肺大鼠肺脏对抗原的免疫应答以及肺泡巨噬细胞吞噬功能 ,探讨矽肺易受感染的免疫学基础。方法 分别两次通过气道、腹腔给予矽肺大鼠模型及正常对照动物卡介苗 (BCG) ,动态检测两次免疫后血清特异性IgG水平 ,试验终末测定肺灌洗液特异性IgG水平 ;皮内注射BCG观察动物迟发型超敏反应 ;气道注入鸡红细胞 ,评价肺泡巨噬细胞的吞噬功能 ;体外检测脾淋巴细胞分泌特异性IgG水平。结果 抗原免疫后矽肺气道 /腹腔免疫组血清与肺灌洗液特异性IgG水平显著高于正常对照组 ,肺泡巨噬细胞吞噬率、吞噬指数显著低于正常对照大鼠 ,迟发性变态反应与正常对照相似。结论 矽肺大鼠对抗原刺激表现出强烈的特异性IgG反应 ,矽肺动物肺泡巨噬细胞吞噬功能显著下降。
OBJECTIVE: To investigate the immunological response of silicotic susceptibility to silicosis by observing the immune response of antigens to lung and the phagocytosis of alveolar macrophages. Methods The silicotic rat model and normal control animal BCG were given twice through the airway and intraperitoneal respectively. The serum IgG levels were detected twice after immunization, and the level of IgG specific to lung lavage fluid was measured at the end of the experiment. The animals were injected with BCG to observe the delayed-type hypersensitivity reaction. The airway was infused with chicken erythrocytes to evaluate phagocytosis of alveolar macrophages. The level of specific IgG secreted by spleen lymphocytes was detected in vitro. Results The specific IgG level of serum and lung lavage fluid after immunization with silico-pulmonary airway / intraperitoneal immunization was significantly higher than that of the normal control group. The phagocytic rate and phagocytic index of alveolar macrophages were significantly lower than those of normal control rats, delayed-type hypersensitivity Similar to the normal control. Conclusion Silicosis rats showed a strong specific IgG response to antigen stimulation, and the phagocytosis of alveolar macrophages in silicosis animals was significantly decreased.