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[目的]大白菜软腐病是影响白菜正常生长的重要病害,严重影响白菜的产量和品质。化学防治是控制大白菜软腐病的重要措施。为了进一步明确甲氧基丙烯酸酯类和抗生素类药剂对大白菜软腐病的防治效果,开展试验研究。[方法]从腐烂大白菜叶片分离得到大白菜软腐病菌(Erwinia carotovora subsp.carotovora),采用16S rDNA序列比对的方法对其进行鉴定。将其作为防治对象,测试杀菌剂的生物活性。[结果]不同药剂对大白菜软腐病菌的生物活性差异很大,同一药剂在离体试验和活体试验中的结果也并不相同。[结论]由于药剂的生物活性测试结果在离体和活体试验中表现出差异性,需要将2种试验方法相结合,才能对药剂生物活性进行准确评价。
[Objective] Chinese cabbage soft rot disease is an important disease that affects the normal growth of Chinese cabbage and seriously affects the yield and quality of Chinese cabbage. Chemical control is an important measure to control soft rot of Chinese cabbage. In order to further clarify the methyoxy acrylates and antibiotics agents on the control of soft rot of cabbage, to carry out pilot studies. [Method] Erwinia carotovora subsp. Carotovora was isolated from rotten cabbage leaves and identified by 16S rDNA sequence alignment. As a control object, test the biological activity of fungicides. [Result] The biological activity of different medicaments to Chinese cabbage soft rot fungus differed greatly. The results of in vitro test and in vivo test of the same medicament were also different. [Conclusion] Because the biological activity test result of the medicaments showed the difference in in vitro and in vivo experiments, the two kinds of test methods need to be combined in order to evaluate the biological activity of the medicaments accurately.