将印楝种子中杀虫活性成分分离成油溶性和水溶性2个混合物后,再用萃取分离法分别将各混合物分离为3个小组,含活性物质个数较多的小组用快速低压液相色谱法分离为124个极性不同的产物,分离产物中的活性物质个数用薄层色谱法鉴定,对有代表性的6个分离产物进行杀虫活性初步试验。结果表明,本试验中,用于提取和萃取印楝种仁中的杀虫活性成分的有机溶剂的搭配是目前较好的一种,能达到油溶性活性成分与油脂基本分离;用快速低压液相色谱法分离的印楝杀虫活性物质具有很高的杀虫选择性和广谱杀虫性。 The neem seed insecticidal active ingredient was separated into two kinds of oil-soluble and water-soluble mixture, and then separated by extractive separation of each mixture into three groups, containing more active substances in the group with a rapid low-pressure liquid phase Chromatography was separated into 124 products with different polarities. The number of active substances in the isolated products was identified by TLC. A preliminary test on the insecticidal activity of 6 representative products was carried out. The results show that in this experiment, the organic solvent used to extract and extract insecticidal active ingredients in neem seed is the better one, which can achieve the basic separation of the oil-soluble active ingredient and fat; The chromatographic separation of neem insecticidal active substance has high insecticidal selectivity and broad-spectrum insecticidal properties.