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选取6份黄瓜品种和2份近缘种(非洲角和酸黄瓜)进行了南方根结线虫病抗性鉴定,2份近缘野生种高抗南方根结线虫病,6份黄瓜品种为感病类型。接种前后根系酶活性与病情指数相关性分析表明,超氧化物歧化酶(SOD)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)活性变化与其病情指数分别呈显著正相关(r=0.8278*)、极显著负相关(r=-0.9679**)和显著负相关(r=-0.8499*)。POD同工酶电泳分析表明,抗病材料酸黄瓜较未接种对照增加了3条酶带(相对迁移率Rf=0.126,0.188,0.944),而感病材料北京截头只增加了1条弱带(Rf=0.396)。SOD、POD及PAL活性水平可作为甜瓜属作物抗根结线虫病鉴定的生化指标及常规抗性鉴定方法的补充。
Six cucumber varieties and two closely related species (African horn and cucumber) were selected to identify the resistance of southern root knot nematodes. Two wild relatives were highly resistant to root knot nematodes. Six cucumber varieties were susceptible Types of. Correlation analysis of root enzyme activity and disease index before and after inoculation showed that the changes of SOD, POD and PAL were positively correlated with their disease index (r = 0.8278 *), a significant negative correlation (r = -0.9679 **) and a significant negative correlation (r = -0.8499 *). POD isozymes electrophoresis analysis showed that the resistant material cucumber increased three bands compared with the uninoculated control (relative mobility Rf = 0.126,0.188,0.944), while the susceptible material Beijing truncated only added a weak band (Rf = 0.396). The activity of SOD, POD and PAL can be used as a biochemical marker for identification of resistance to root-knot nematode disease in melon genus and the supplement of routine resistance identification methods.