论文部分内容阅读
目的探讨外源性肾上腺髓质素(ADM)在大鼠肾脏急性机械性损伤早期对血管紧张素Ⅱ(Ang-Ⅱ)、丙二醛(MDA)表达的影响及对肾损伤的保护作用。方法健康成年普通级Wister大鼠104只,随机分为正常对照组(8只)、单纯创伤组(32只)、伤前给药组(32只)、伤后给药组(32只);后3组采用自由落体打击仪打击大鼠脊肋区制作肾机械性损伤模型。伤前、伤后给药组分别于打击前后10min腹腔注射ADM(0.1nmol/kg)。各组大鼠平均分为4批,分别在肾脏机械打击后1、6、12、24h处死。提取各组肾组织匀浆上清液,利用放免分析法检测Ang-Ⅱ浓度,比色法检测MDA浓度。结果 Ang-Ⅱ浓度:单纯创伤组在创伤早期(1、6h)明显高于正常对照组(P<0.05);伤前给药组1、6h,伤后给药组1h均低于同时间点单纯创伤组(P<0.05)。MDA浓度:单纯创伤组在创伤1h高于正常对照组(P<0.05);伤前给药组6h,伤后给药组12h均低于同时间点单纯创伤组(P<0.05)。结论外源性ADM通过降低Ang-Ⅱ与MDA的浓度而对急性创伤肾组织发挥保护作用,其中创伤前注射ADM对肾组织损伤的抑制作用优于创伤后注射ADM。
Objective To investigate the effect of exogenous adrenomedullin (ADM) on the expression of angiotensin Ⅱ (Angiotensin Ⅱ) and malondialdehyde (MDA) and its protective effect on renal injury in the early stage of renal acute mechanical injury in rats. Methods Totally 104 healthy adult Wister rats were randomly divided into normal control group (n = 8), trauma group (n = 32), pre - injury group (n = 32) and post - traumatic injury group (n = 32) The latter three groups were subjected to a mechanical injury model of kidney by using a free fall bashing machine against the rib cage of rats. Injured before and after injury group were administered intraperitoneally ADM (0.1nmol / kg) 10min before and after the blow. The rats in each group were divided into 4 batches on average, and were sacrificed at 1, 6, 12 and 24 hours after the kidney was mechanically challenged. The supernatant of renal homogenate of each group was extracted, the concentration of Ang-Ⅱ was detected by radioimmunoassay, and the concentration of MDA was detected by colorimetry. Results The concentration of Ang-Ⅱ in trauma group was significantly higher than that in normal control group (P <0.05) at 1 h and 6 h after trauma, Simple trauma group (P <0.05). The concentration of MDA in trauma group was higher than that in normal control group at 1 hour after trauma (P <0.05). The trauma group at 6 hours after trauma and 12 hours after trauma group were lower than those at trauma group at the same time point (P <0.05). Conclusions Exogenous ADM can protect renal tissue from acute trauma by decreasing the concentration of Ang-Ⅱ and MDA. The pretreatment ADM is superior to traumatic ADM in inhibiting renal tissue injury.