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昆虫杆状病毒表达系统以其表达产量高、表达产物理化和生物特性与天然蛋白相似等优点 ,成为目前较理想的真核表达系统。该系统的主要步骤包括亲本病毒的培养鉴定、病毒 DNA的纯化、重组转移载体的构建、共转染形成重组病毒及重组病毒感染昆虫细胞表达目的蛋白。其中 ,病毒培养?
The insect baculovirus expression system has become an ideal eukaryotic expression system for its high yield of expression, physical and chemical properties of the expressed product and similarities with the natural protein. The main steps of the system include the cultivation and identification of the parent virus, the purification of the virus DNA, the construction of a recombinant transfer vector, co-transfection to form the recombinant virus and the recombinant virus to infect insect cells to express the target protein. Among them, the virus culture?