食用菌的遗传分析工作在国内外几近空白,为使食用菌遗传育种工作建立在坚实的基础上,我们进行了本实验,现将初步结果报道如下: 一、材料与方法 (一)供试菌株 佛罗里达侧耳营养缺陷型突变体,用DES化学诱变获得,具体方法参见《食用菌》1992年第6期“佛罗里达侧耳担孢子的化学诱变”一文。 (二)培养基 a.基本培养基(MM):葡萄糖2％,硝酸钠0.4％,磷酸二氢钾0.1％,硫酸镁0.05％,氯化钾0.05％,琼脂1.5％,硫酸亚铁、硫酸锌、硫酸铜各微量,重蒸馏水配制,pH值自然。b.完全培养基(CM):葡萄糖2％,蛋白胨0.4％,磷酸二氢钾0.05％,硫酸镁0.05％,碳酸钙0.01％,琼脂1.5％,pH值自然。以上均为8磅20分钟灭菌。c.出菇培养 Genetic analysis of edible mushrooms at home and abroad almost blank, in order to make edible mushroom genetic breeding work on a solid foundation, we conducted this experiment, the preliminary results are reported as follows: First, materials and methods (A) for the test Strain Florida lateral ear auxotrophic mutant obtained by DES chemical mutagenesis, the specific method, see “Edible Fungus” 1992 the sixth “Florida side of the spores of the chemical mutagenesis” article. (B) Medium a. Basic medium (MM): glucose 2%, sodium nitrate 0.4%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, potassium chloride 0.05%, agar 1.5%, ferrous sulfate, Zinc, copper sulfate trace, heavy distilled water preparation, pH value of natural. b. Complete medium (CM): 2% glucose, peptone 0.4%, potassium dihydrogen phosphate 0.05%, magnesium sulphate 0.05%, calcium carbonate 0.01%, agar 1.5%, pH natural. All above are sterilized at 8 pounds for 20 minutes. c. fruiting culture