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利用不同紫外线照射时间和不同浓度秋水仙素浸种的方法对水稻陈年种子进行处理后,通过测定其萌发率和淀粉酶活性来分析两种因子的影响。结果显示:1、紫外线照射2 h以上,可极显著降低种子萌发率,但是0.2%和0.3%浓度秋水仙素则能显著提高萌发率。两种因子不同组合处理均能极显著地促进萌发率,其中3个处理组的萌发率都高达92.22%。有秋水仙素处理并且紫外线照射2 h以上种子的萌发率并不下降。2、不同时间紫外照射都能使萌发中淀粉酶活性极显著地提高。0.2%和0.4%的秋水仙素处理使酶活性显著地提高,0.3%和0.5%浓度处理的效果极显著地高于对照组。两因子分别处理时,秋水仙素处理的效果没有紫外线好。两种因子不同组合的处理中有10个组的酶活性显著提高。大多数处理组酶活性呈现出两个高峰值,分别出现在第二天和第五天,所有的处理组均在第一天或第二天达到酶活性的最大值。
The effects of two factors were analyzed by measuring the germination rate and amylase activity of aged seeds treated with different UV irradiation times and different concentrations of colchicine soaking seeds. The results showed that: 1, UV irradiation for more than 2 h, can significantly reduce the seed germination rate, but 0.2% and 0.3% colchicine can significantly improve the germination rate. The combination of two factors could significantly promote the germination rate, of which the three treatment groups germination rate as high as 92.22%. With colchicine treatment and ultraviolet radiation 2 h above the seed germination rate does not decline. 2, UV irradiation at different times can make germination amylase activity was significantly improved. Colchicine treatments of 0.2% and 0.4% increased the enzyme activity remarkably, and the effects of 0.3% and 0.5% treatments were significantly higher than those of the control group. When treated with two factors, colchicine treatment did not UV good. The enzyme activity of 10 groups increased significantly in different combinations of two factors. The enzyme activity of most treatment groups showed two peak values, which appeared on the second day and the fifth day respectively, and all the treatment groups reached the maximum value of enzyme activity on the first day or the second day.