作者以从绿脓杆菌粘性 385株分离纯化得到的 6 0 k Da的过氧化氢酶同工酶 A( Kat A)为候选疫苗 ,用急性呼吸道绿脓杆菌感染大鼠模型对 Kat A的免疫原性和保护作用进行研究。　　用 Zwittergent抽提法粗提 Kat A后 ,再用 Bio- scale Q5离子交换层析柱和制备用十二烷基硫酸钠 -聚 In this paper, we used a 60 kDa catalase A (Kat A) isolated and purified from 385 strains of Pseudomonas aeruginosa as a candidate vaccine, and used the acute respiratory tract Pseudomonas aeruginosa infected rat model to test the immunogenicity of Kat A Sexual and protective effects of research. Crude extract Kat A extraction with Zwittergent, and then use Bio-scale Q5 ion exchange chromatography and preparative sodium lauryl sulfate - poly