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根据Hertwig效应,采用正交设计实验方法对真鲷(Pagrus major)冷冻精子诱导大菱鲆(Scophthal-mus maximus)减数分裂雌核发育的最佳条件进行了研究.结果表明,未经紫外线照射处理的真鲷精子与大菱鲆卵子受精后形成的胚胎,无论是否经过冷休克处理都不能孵化出膜;经剂量为64.8～72.0mJ.cm-2紫外线照射处理后的真鲷精子与大菱鲆卵子受精后形成的胚胎,初孵仔鱼表现出典型的单倍体综合征;受精和孵化水温为(14.5±0.5)℃时,大菱鲆雌核发育单倍体的冷休克二倍体化的最佳处理起始时间、处理水温和持续时间分别为受精后6.5min,-2℃和45min.至孵化后60日龄,雌核发育苗种的成活率为0.61%,仅相当于其半同胞对照的10%左右,60～180日龄两者的成活率差异不显著,均达90%以上;14月龄前,雌核发育苗种的全长和体重均比半同胞对照要低,此后雌核发育苗种由于雌性比例较高而生长速度加快,至17月龄时其全长和体重与半同胞对照基本相当.
According to the Hertwig effect, orthogonal design was used to study the optimal conditions for gynogenetic meiosis of Scophthal-mus maximus induced by frozen spermatozoa in Pagrus major. The results showed that without UV irradiation The embryos formed after sperm and turbot turbid eggs fertilized could not hatch out of the membrane even after cold shock treatment. After treated with a dose of 64.8 ~ 72.0 mJ · cm-2, Paralichthys olivaceus embryos and newly hatched larvae showed typical haploid syndromes. Cold shock diploidization of the haploid gynogenes of turbot was performed at the fertilization and incubation temperature of (14.5 ± 0.5) ℃ The optimal treatment start time, treatment water temperature and duration were respectively 6.5min, -2 ℃ and 45min after fertilization.Through 60 days after hatching, the survival rate of gynogenetic seed was 0.61% There was no significant difference in the survival rate between 60 and 180 days old when the control was about 10%, all reaching over 90%. Before the age of 14 months, the total length and body weight of gynogenetic seedlings were lower than that of the half-siblings, Nuclear seedlings grow faster due to the higher proportion of females At 17 months of age its full length and body weight were comparable to those of the half-sib.