论文部分内容阅读
目的：克隆大鼠肝脏再生增强因子（ＡＬＲ）ｃＤＮＡ，并对其序列进行分析。方法：建立大鼠２／３肝切除模型，从再生肝组织中提取总ＲＮＡ，利用ＲＴ－ＰＣＲ技术扩增出大鼠ＡＬＲｃＤＮＡ，亚克隆于ｐＧＥＭ－Ｔ载体，并将ＤＮＡ序列及其推测的氨基酸序列与Ｇｅｎｅｂａｎｋ作同源性比较。结果和结论：克隆到大鼠ＡＬＲｃＤＮＡ，经核苷酸序列测定证实序列完全正确，其核苷酸序列没有任何突变，它与酵母ＥＲＶ１ｃＤＮＡ有较高的同源性，核苷酸序列同源性达５４．９９％，氨基酸序列同源性达４７．０１％，并发现氨基酸序列中含有锌指蛋白结合区域。
OBJECTIVE: To clone rat liver regeneration augmentation factor (ALR) cDNA and analyze its sequence. Methods: The rat model of 2/3 hepatectomy was established. The total RNA was extracted from the regenerated liver tissue. The rat ALR cDNA was amplified by RT-PCR and subcloned into pGEM-T vector. The DNA sequence and its deduced amino acid Homology comparison of sequences with Genebank. RESULTS AND CONCLUSION: The rat ALR cDNA was cloned and verified by nucleotide sequence analysis. The nucleotide sequence of the rat ALR cDNA was confirmed to be correct. The nucleotide sequence of the rat ALR cDNA did not have any mutation. The nucleotide sequence homology with the yeast ERV1 cDNA was very high. 54.99%, the amino acid sequence homology was 47.01%, and the amino acid sequence was found to contain the zinc finger protein binding region.