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探讨长期或反复使用链霉素所至的耳、肾毒性与机体链霉素抗体间的关系。健康豚鼠用SM -BSA结合物、BSA分别免疫后 ,再注射不同剂量的纯链霉素30d ,测定其听觉脑干电反应的变化 ,组织切片HE染色和免疫组织化学染色 ,观察内耳功能及内耳和肾的形态变化。研究结果表明 ,未免疫只用不同剂量的链霉素组的豚鼠内耳听觉功能及内耳和肾的形态变化甚小。用BSA免疫后再用不同剂量的链霉素组豚鼠上述器官有一定程度的损伤 ,而用SM -BSA结合物免疫后再用不同剂量的链霉素组豚鼠的听力下降明显 ,内耳螺旋器 (Corti器 )有典型的毛细胞损伤表现而且损伤程度有一定的剂量依赖性 ,肾皮质部肾小管出现轻度的形态改变 ,肾小管上皮细胞内出现颗粒状沉积物 ,免疫组织化学染色呈阳性。长期或反复用链霉素后其肾和内耳毒性作用的出现与加重与其抗原抗体免疫复合物的形成有关。
To investigate the long-term or repeated use of streptomycin to the ear, nephrotoxicity and the relationship between streptomycin antibody. The healthy guinea pigs were immunized with SM-BSA conjugate and BSA respectively, and then different streptomycin doses were injected for 30 days. The changes of auditory brainstem response were observed. HE staining and immunohistochemistry were used to observe the inner ear function and inner ear And the morphological changes of the kidneys. The results showed that the non-immunized guinea pigs with different doses of streptomycin had no significant changes in their auditory function and morphology of the inner ear and kidney. After immunization with BSA, different doses of streptomycin were used to induce the above organs to some extent in guinea pigs. However, the hearing loss of guinea pigs with different doses of streptomycin after immunization with SM-BSA conjugate was significantly decreased. Corti) showed typical hair cell damage and showed a certain degree of damage in a dose-dependent manner. The renal cortical tubules showed slight morphological changes, granular deposits in renal tubular epithelial cells, and immunohistochemical staining was positive. Long-term or repeated use of streptomycin after the toxic effects of its kidney and inner ear appear and aggravate the formation of its antigen-antibody immune complexes.