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目的 研究HER - 2 /neu(c -erbB - 2 )在卵巢上皮性肿瘤中的表达及意义。方法 收集 10 6例卵巢上皮性肿瘤 (恶性 5 4便 ,交界性 33例 ,良性 19例 )及临床资料。按国际妇产科联合会(FIGO)标准进行分期 ,卵巢中Ⅰ、Ⅱ期共 19例 (19/ 5 4 ,35 .2 % ) ,Ⅲ、Ⅳ期 35例 (35 / 5 4 ,6 4 .8% ) ,交界性肿均为Ⅰ期。采用标记链霉素卵白素生物素 (LSAB)法用HER - 2 /neu多克隆抗体 (DAKO ,A0 4 85 )行免疫组织化学染色。结果 该蛋白表达的阳性率在良性肿瘤为 4 7.4 % (9/ 19) ,过表达 (3+)为 0 ;交界性肿瘤为 84 .8% (2 8/ 33) ,过表达率为 9.1% (3/ 33) ;恶性肿瘤为 85 .2 % (46 / 5 4 ) ,过表达率为 2 5 .9%(14 / 5 4 )。良性与交界性以及良性与恶性之间的HER - 2 /neu蛋白表达率的差异有显著性 (P〈 0 .0 2 ,P〈0 .0 1) ;该蛋白的过表达率在有转移和无转移的病例之间差异有显著性意义 (P〈0 .0 0 1)结论 HER- 2 /neu的过表达与卵巢癌的侵袭性生物学行为相关
Objective To study the expression and significance of HER-2 / neu (c-erbB-2) in epithelial ovarian tumors. Methods A total of 106 cases of ovarian epithelial tumors (54 cases of malignancy, 33 cases of borderline and 19 cases of benign disease) and clinical data were collected. According to the standard of the International Federation of Gynecology and Obstetrics (FIGO), there were 19 cases (19/54, 35.2%) in stages I and II and 35 cases (35/54, 64) in stages III and IV. 8%), borderline swollen are phase Ⅰ. Immunohistochemical staining with HER-2 / neu polyclonal antibody (DAKO, A0 4 85) was performed using the labeled streptavidin biotin (LSAB) method. Results The positive expression rate of this protein was 4 7.4% (9/19) in benign tumors and 0 in overexpression (3+). The positive rate of borderline tumors was 84.8% (28/33) and the overexpression rate was 9.1% (3/33), malignant tumor was 85.2% (46/544), and the overexpression rate was 25.9% (14/54). There was a significant difference in the expression rates of HER - 2 / neu between benign and borderline and between benign and malignant (P <0. 02, P <0.01). The overexpression rate of HER - 2 / There was a significant difference between cases without metastasis (P <0.001) Conclusions The overexpression of HER-2 / neu is associated with aggressive biological behavior in ovarian cancer