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初步建立了一种分离纯化人腮腺液蛋白的方法。探讨了样品的预处理、不同固定相、流动相以及不同检测波长等因素对正常人腮腺液蛋白疏水色谱分离的影响。结果在40min梯度内,以2mol/L硫酸铵为A流动相,以0.05mol/L磷酸二氢钾为B流动相(100%A∶0%5~0%A∶100%B),腮腺液蛋白被分离出6个峰。经生化方法证实,用疏水色谱分离人唾液蛋白的方法,分离度好,保持了蛋白样品的生物活性,且样品处理简单,可成为研究人唾液蛋白成分的一种较理想的方法。
A preliminary method for the isolation and purification of human parotid gland fluid protein was established. The effects of sample pretreatment, different stationary phase, mobile phase and different detection wavelength on the hydrophobic chromatographic separation of parotid gland fluid were investigated. Results In a gradient of 40 min, the mobile phase consisted of 2mol / L ammonium sulfate as mobile phase A, 0.05mol / L potassium dihydrogen phosphate as mobile phase B (100% A: 5% to 0% A: 100% B) The liquid protein is separated into six peaks. Biochemical methods confirmed that the separation of human saliva protein by hydrophobic chromatography, good resolution, to maintain the biological activity of protein samples, and sample processing is simple, can be a more ideal method for the study of human saliva protein components.