论文部分内容阅读
目的研究类风湿关节炎(rheumatoidarthritis,RA)患者病灶部位即关节滑膜组织浸润的受某种自身抗原驱动的T淋巴细胞抗原受体(Tcellreceptor,TCP)“链的取用格局。方法采用实时定量聚合酶链反应(polymerasechainreaction,PCR)的方法分析RA患者病灶部位T淋巴细胞抗原受体”链的取用;采用PCR-SSP方法对所调查的RA患者的人类白细胞抗原(humanleucocyteantigen,HLA)进行了分型。结果研究发现中国人群RA患者病灶关节滑膜浸润的T淋巴细胞抗原受体主要取用BV14和BV16家族;中国人群RA患者HLA基因型主要以HLADRB1*0405为主要特征,与白种人RA患者不同。结论研究提示中国人群RA患者病灶区自身反应性T细胞具有TCRBV14和BV16的限制性取用,其中TCRBV16的偏移为国内外首次报道。而HLADRB1*0405类风湿关节炎患者表现出TCRBV16取用的趋势,提示TCRBV16取用受主要组织相容性复合体(MHC)的约束,这为进一步分析诱发RA的发生的免疫异常和自身反应性T细胞的生物学特性提供了重要试验基础。
Objective To study the pattern of T cell receptor (Tcll receptor) driven by some autoantigen in the synovial tissue of patients with rheumatoid arthritis (RA) .Methods Real-time Quantitative polymerase chain reaction (PCR) method was used to analyze the T lymphocyte antigen receptor chain in the RA patients. PCR-SSP was used to detect the human leucocyte antigen (HLA) Have been typed. Results The study found that the main receptor of T lymphocyte antigen of synovial infiltration in Chinese RA patients was BV14 and BV16 families. HLA genotypes of RA patients in Chinese population were mainly characterized by HLADRB1 * 0405, which was different from that of Caucasian RA patients . Conclusions The study suggests that TCRBV14 and BV16 are restricted access to autoreactive T cells in the lesion region of RA patients in China. The shift of TCRBV16 is the first reported in China and abroad. However, patients with HLADRB1 * 0405 rheumatoid arthritis showed a trend of taking TCRBV16, suggesting that TCRBV16 access is restricted by the major histocompatibility complex (MHC), which may be helpful to further analyze the immune abnormalities and auto-reactivity induced by RA The biological characteristics of T cells provide an important experimental basis.