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目的:探讨食管鳞状细胞癌组织中血管、淋巴管生长特点与细胞增殖关系的临床病理意义。方法:应用免疫组化PicTure-Plus法检测62例食管鳞癌石蜡包埋组织Ki-67、CD34和D2-40表达,计数癌巢及其周围功能血管密度(BVD)、微血管密度(MVD)及淋巴管密度(LVD)。结果:62例食管鳞癌组织血管、淋巴管的生长分布存在明显的不均一性,癌周明显多于癌巢组织,且其生长具有明显异质性。MVD在食管下段癌中明显多于中、上段癌,且随组织分化程度的降低而增加、随浸润深度的增加而增加、随S分期的增加而增加,P均<0.05;LVD在淋巴结有转移组明显多于无转移组,SⅢ期明显多于SⅠ期,P均<0.05。Ki-67表达与癌周MVD、BVD和LVD呈正相关,P均<0.05;D2-40在食管鳞癌细胞质中亦有表达,且与癌巢中的MVD、BVD呈正相关,P均<0.05。结论:食管鳞癌组织中癌细胞的增殖与癌周血管、淋巴管的生长呈正相关,血管、淋巴管过度生长是临床易进展的标志之一;Ki-67、D2-40均可作为预后判断的独立检测指标。
Objective: To investigate the clinicopathological significance of the relationship between the growth characteristics of vascular and lymphatic vessels in esophageal squamous cell carcinoma and cell proliferation. Methods: The expressions of Ki-67, CD34 and D2-40 in paraffin-embedded tissues of 62 cases of esophageal squamous cell carcinoma were detected by immunohistochemical method of PicTure-Plus. The function of the neoplasm and its surrounding functional vessel density (BVD), microvessel density (MVD) Lymphatic vessel density (LVD). Results: 62 cases of esophageal squamous cell carcinoma blood vessels and lymphatic vessels were significantly heterogeneous growth distribution, significantly more than the week of cancerous neoplasms, and its growth has obvious heterogeneity. MVD was significantly higher in the lower esophageal cancer than in the upper and upper cancer, and increased with the degree of differentiation, increased with the depth of invasion, increased with the increase of S staging, P <0.05; LVD in the lymph node metastasis Group was significantly more than without metastasis, S Ⅲ was significantly more than S Ⅰ, P all <0.05. The expression of Ki-67 was positively correlated with the MVD, BVD and LVD of cancer, P <0.05 respectively. D2-40 was also expressed in the cytoplasm of esophageal squamous cell carcinoma, and positively correlated with MVD and BVD in cancer nests (P <0.05). Conclusion: The proliferation of cancer cells in esophageal squamous cell carcinoma is positively correlated with the growth of perivascular and lymphatic vessels. The overgrowth of blood vessels and lymphatic vessels is one of the markers of clinical progression. Ki-67 and D2-40 can be used as prognostic factors The independent test indicators.