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目的:建立测定人血浆中亮菌甲素琥珀酸单酯的高效液相色谱法。方法:色谱柱为DL-C_(18)(150 mm×4.6 mm,5μm),流动相:甲醇-10 mmol乙酸铵水溶液-乙酸(45:55:0.5);柱温25℃,流速1.0ml·min~(-1),检测波长220 nm。结果:亮菌甲素琥珀酸单酯方法最低检测浓度为5 ng·ml~(-1),线性范围0.05～10.00μg·ml~(-1)(r=0.999 6)线性关系良好。亮菌甲素琥珀酸单酯日内RSD分别为2.09%～4.22%,日间RSD分别为3.72%～5.04%。方法回收率分别为94.38%～97.20%。结论:本试验建立的方法简便、快速、准确,适用于亮菌甲素琥珀酸单酯体内血药浓度和生物等效性的测定。
OBJECTIVE: To establish a HPLC method for the determination of strychnine succinate in human plasma. Methods: The chromatographic column was DL-C 18 (150 mm × 4.6 mm, 5 μm). The mobile phase was methanol-10 mmol ammonium acetate aqueous solution-acetic acid (45:55:50) min ~ (-1), detection wavelength 220 nm. Results: The minimum detectable concentration of strychnine succinate monoester was 5 ng · ml ~ (-1) and the linear range was 0.05 ~ 10.00 μg · ml ~ (-1) (r = 0.999 6). The intradermic RSD of sreptomycin succinic acid monoester was 2.09% ~ 4.22%, respectively. The intraday RSD was 3.72% ~ 5.04%. The recoveries were 94.38% ~ 97.20%. Conclusion: The method established in this study is simple, rapid and accurate and is suitable for the determination of plasma concentration and bioequivalence of strychnine succinate monoester in vivo.